Antioxidants Improve the Proliferation and Efficacy of hUC-MSCs against H2O2-Induced Senescence

Human umbilical cord mesenchymal stem cells (hUC-MSCs) are broadly applied in clinical treatment due to convenient accessibility, low immunogenicity, and the absence of any ethical issues involved. However, microenvironment inflammatory tissues may cause oxidative stress induce senescence transplanted hUC-MSCs, which will further reduce proliferation, migration ability, final therapeutic effects hUC-MSCs. Beta-nicotinamide mononucleotide (NMN) coenzyme Q10 (CoQ10) famous antioxidants longevity medicines that could intracellular reactive oxygen species levels by different mechanisms. In this study, hUC-MSCs were treated vitro with NMN CoQ10 determine if they caused hydrogen peroxide (H2O2) recover cell functions. The on mRNA cytokine TNFα anti-inflammatory IL10, differentiation ability before after H2O2 investigated. findings revealed reduced H2O2-induced increased hUC-MSCs’ proliferation late phase as passage 12 later. level induced was significantly decreased antioxidant treatment. all adipogenic improved chondrogenic Furthermore, found enhance Transcriptomic analysis both DNA repair cyclin expression downregulated TNF IL-17 signaling pathways, thereby contributing proliferative promotion senecent resistance stress. These suggest can improve survival efficacy therapy for inflammation-related diseases.